Juventus want to defend their perfect start with new star signings on the bench, while Roma seek their first victory with surprise changes, including debutant Alexis Saelemaekers.
It kicks off at the Allianz Juventus Stadium in Turin at 19.45 UK time (18.45 GMT).
You can follow all the build-up and action as it happens from all today’s Serie A games on the LIVEBLOG.
The Bianconeri could not have asked for a better start to the Thiago Motta era, winning both games 3-0, but this is their first major test.
Tim Weah, Khephren Thuram and Arkadiusz Milik are out of action, but new signings Nico Gonzalez, Teun Koopmeiners, Francisco Conceicao, Pierre Kalulu and Douglas Luiz are on the bench.
The coach keeps faith with Next Gen products Nicolò Savona and Samuel Mbangula, who both scored on their full senior debuts.
Kenan Yildiz is in a trequartista role supporting Dusan Vlahovic.
Roma had a complicated campaign, drawing at Cagliari and suffering a shock home defeat to Empoli, all in the middle of transfer chaos around Dybala and failed medicals for Tiago Djalo and Kevin Danso.
Manu Kone is on the bench following his deadline day move from Borussia Monchengladbach, while Enzo Le Fée is injured.
Still trying to find their shape with so many new players, Daniele De Rossi had been expected to move to a 3-5-2 with Saelemaekers given his debut from the start behind Dybala and Artem Dovbyk, leaving Matias Soulé on the bench against his old club.
Instead, it is a 4-3-3 with Dybala and Leandro Paredes dropped to the bench in favour of 19-year-old Niccolo’ Pisilli’s first ever senior start.
Juventus vs Roma – how to watch on TV
Juventus-Roma is on Paramount+ in the USA. Fans in the UK and Ireland can watch it on TNT Sports or OneFootball for £4.99. beIN SPORTS 1 or beIN SPORTS CONNECT are your places to go if you want to watch the game from Australia or New Zealand.
Juventus vs. Roma line-ups
Juventus: Di Gregorio; Savona, Bremer, Gatti, Cabal; Locatelli, Fagioli; Cambiaso, Yildiz, Mbangula; Vlahovic
Rome: Svilar; Celik, Mancini, Ndicka, Angelino; Pisilli, Cristante, Pellegrini; Soulé, Dovbyk, Saelemaekers
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